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Table 3 Experiment design of steepest ascent (descent) and corresponding response

From: Cost-effective production of alginate oligosaccharides from Laminaria japonica roots by Pseudoalteromonas agarivorans A3

Experiment number

NH4NO3 (%)

Fermentation temperature (°C)

pH

Inoculum size (%)

Enzyme activity (U/ml)

1

1.5

22.5

7.5

1.25

2.36

2

1.75

21

7.75

1.5

4.42

3

2

19.5

8.0

1.75

8.56

4

2.25

18

8.25

2

8.28

5

2.5

16.5

8.5

2.25

0.16

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Microbial Cell Factories

ISSN: 1475-2859

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