Table 2 Plackett–Burman (PB) design for screening of culture conditions affecting the alginate lyase production of strain A3
Run | X1 | X2 | X3 | X4 | X5 | X6 | X7 | X8 | X9 | X10 | X11 | Enzyme activity (U/ml) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 1.2 | 180 | 0.4 | 36 | 25 | 7 | 2 | 3 | 1 | − 1 | − 1 | 1.10 |
2 | 1.2 | 120 | 0.6 | 24 | 25 | 8 | 1 | 3 | 1 | 1 | − 1 | 1.51 |
3 | 1.2 | 120 | 0.4 | 36 | 20 | 8 | 2 | 2 | 1 | 1 | 1 | 4.37 |
4 | 1.8 | 180 | 0.6 | 24 | 20 | 7 | 2 | 2 | 1 | 1 | − 1 | 4.11 |
5 | 1.8 | 120 | 0.4 | 24 | 25 | 7 | 2 | 3 | − 1 | 1 | 1 | 1.39 |
6 | 1.8 | 180 | 0.4 | 36 | 25 | 8 | 1 | 2 | − 1 | 1 | − 1 | 2.02 |
7 | 1.8 | 120 | 0.6 | 36 | 25 | 7 | 1 | 2 | 1 | − 1 | 1 | 1.26 |
8 | 1.2 | 180 | 0.6 | 24 | 25 | 8 | 2 | 2 | − 1 | − 1 | 1 | 2.24 |
9 | 1.2 | 180 | 0.6 | 36 | 20 | 7 | 1 | 3 | − 1 | 1 | 1 | 3.59 |
10 | 1.8 | 180 | 0.4 | 24 | 20 | 8 | 1 | 3 | 1 | − 1 | 1 | 4.42 |
11 | 1.2 | 120 | 0.4 | 24 | 20 | 7 | 1 | 2 | − 1 | − 1 | − 1 | 2.46 |
12 | 1.8 | 120 | 0.6 | 36 | 20 | 8 | 2 | 3 | − 1 | − 1 | − 1 | 6.67 |