Fig. 4
The alginate lyase production of strain A3 cultured under the conditions before and after optimization. Before optimization, strain A3 was cultured in the medium (pH 8.0) containing 1% (w/v) L. japonica root powder and 3% sea salts with 1% inoculum size at 20 °C, 180 rpm for 24 h. After optimization, strain A3 was cultured in the medium (pH 8.04) containing 2.03% NH4NO3, 0.6% L. japonica root powder and 3% sea salt with 2% inoculum size at 19.6oC, 120 rpm for 36 h. The graphs show data from triplicate experiments (mean ± S.D.)