Fig. 10

Hematoxylin and eosin (H&E)-stained paw sections [×100]. A Group I (negative control) showed normal skin with epidermis of average thickness (black arrow), thick keratin (blue arrow) and underlying normal dermis (red arrows). B Group II (positive control) showed surface ulceration (red arrow) with underlying granulation tissue containing congested blood vessels (black arrow) with infiltration by inflammatory cells (blue arrows). C Group III (rats pre-treated with free extract 25 mg) showed mild dermal inflammation (blue arrow), the epidermis was mild thickened lined with mild keratosis (red arrows) with no collagenosis (black arrow). D Group IV (rats pre-treated with free extract 50 mg) showed few dermal inflammation (red arrow), the epidermis was thickened lined with excessive keratosis (black arrows) with mild collagenosis (blue arrow). E Group V (rats pre-treated with Se NPs loaded with extract 25 mg) showed no dermal inflammation; the epidermis was thickened lined with marked keratosis (red arrows) and moderate collagenosis (blue arrow). F Group VI (rats pre-treated with Se NPs loaded with extract 50 mg) showed no inflammation; the epidermis is thickened with excessive keratosis (black arrows) and excessive collagenosis (blue arrow). G Group VII (rats received free extract 50mg only) showed normal skin with epidermis of average thickness (black arrow), thick keratin (blue arrow) and normal dermis (red arrows). H Group VIII (rats received placebo SeNPs only) showed normal skin with epidermis of average thickness (black arrow), thick keratin (blue arrow) and normal dermis (red arrows). I Group IX (rats pre-treated with placebo SeNPs) showed surface ulceration (blue arrow) with underlying granulation tissue (black arrows) and infiltration of inflammatory cells (red arrows)