Table 1 Characterization of primers used in PCR
Primer | Sequence | Length of the starter [nt] | Tm [°C] |
|---|---|---|---|
Fn3 | 5′-CAGGATTAGATACCCTGGTAGTCC-3′ | 24 | 57.4 |
F4 | 5′-CCGCCTGGGGAGTACG-3′ | 16 | 53.6 |
Fn5 | 5′-ACTCCTACGGGAGGCAGCAG-3′ | 20 | 57.9 |
Fn6 | 5′-CCAGCAGCCGCGGTAATAC-3′ | 19 | 55.4 |
Rn1 | 5′-GGCTACCTTGTTACGACTTC-3′ | 20 | 51.8 |
Rn2 | 5′-TGACGGGCGGTGTGTACAAG-3′ | 20 | 55.9 |
Rn3 | 5′-GGCGTGGACTACCAGGGTATC-3′ | 21 | 58.3 |