Table 5 Optically pure AC and BD were obtained by resting cells and complex enzyme transformations
Strains | Methods | Substrate | Product | Enantiomeric excess (%) | Reference |
|---|---|---|---|---|---|
E. coli HB101 | Expression of gdh from Hansenula polymorpha Dl-1 | (2R,3R)-2,3-BD | 3R-AC | > 99.9 | (30) |
E. coil BL21(DE3) | Expression of bdhA from B. subtilis 168 and nox from L. brevis CICC 6004 | (2R,3R)-2,3-BD | 3R-AC | 96 | (31) |
E. coli | Expression of budC from C. glutamicum ATCC13032 and xyl1 from Candida tenuis | meso-2,3-BD | 3R-AC | / | (32) |
E. coli BL21 (DE3) | Co-expression of budC, nox and vhb from Serratia sp. T241 | meso-2,3-BD | 3R-AC | 97.89 | (33) |
E. coli Rosetta | Overexpression of dar from P. polymyxa ZJ-9 | DA | 3S-AC | > 99.9 | (34) |
E. coli BL21(DE3) | Expression of dar with high stereoselectivity, specificity and stability and the in situ-NADH regeneration system | DA | 3S-AC | 99.5 | (35) |
K. pneumoniae CICC 10,011 and B. subtilis 168 | Â K. pneumoniae CICC 10,011 resting cells convert glucose to a mixture of meso-2,3-BD and (2Â S,3Â S)-2,3-BD. B. subtilis 168 resting cells convert meso-2,3-BD in the mixture to 3Â S-AC | Glucose | 3S-AC | 96.2 | (36) |
E. coli BL21(DE3) | Expressing bdhA from B. subtilis 168 and nox from L. brevis CICC 6004 | (2R,3R)-2,3-BD | 3R-AC | 96 | (31) |
meso-2,3-BD | (2S,3S)-2,3-BD | 99 | |||
E. coli | Expressing dar from Enterobacter cloacae sp. | DA | (2S,3S)-2,3-BD | > 99 | (13) |