Table 4 Optically pure AC and BD were obtained by heterologous expression
Strains | Methods | Product | Enantiomeric excess (%) | Reference |
|---|---|---|---|---|
E. coli DH5α | Expression of budRAB and nox in E. coli DH5α | 3R-AC | 97.3 | (19) |
C. glutamicum | Deletion of budC, pta, ack, ldh, nagD, and overexpression of alsS, alsD in C. glutamicum | 3R-AC | > 90 | (20) |
Pichia pastoris | Codon optimized alsS and alsD from B. subtilis and bdhA from S. cerevisiae were integrated into Pichia pastoris genome and expressed under the control of GAP promoter. | (2R,3R)-2,3-BD | > 99 | (21) |
E. coli | A pathway for synthetic optically pure (2R,3R)-2,3-BD was constructed in E. coli | (2R,3R)-2,3-BD | 99 | (22) |
E. coli | Expression of alsS, alsD and budC from K. pneumoniae in E. coli | meso-2,3-BD | 100 | (23) |
S. cerevisiae | Deletion of bdhA and insert budC from K. oxytoca in S. cerevisiae | meso-2,3-BD | / | (24) |
E. coli JM109 | The genes including ldhA, pta, adhE and poxB were deleted from E. coli JM109 and cultured under hypoxic conditions. | meso-2,3-BD | / | (25) |
E. coli YYC202 | Overexpression of ilvC, aldB and bdhA | meso-2,3-BD | / | (26) |
E. coli | Expression genes of budB and budC from Enterobacter cloacae | (2S,3S)-2,3-BD | 95 | (27) |
E. coli | Overexpression of gdh and fdh in E. coli | (2S,3S)-2,3-BD | / | (28) |
L. lactis | Expression gene of budC from E. cloacae in L. lactis | (2S,3S)-2,3-BD | 100 | (29) |