Table 3 Optically pure AC and BD were obtained by editing wild strains
Strains | Methods | Product | Enantiomeric excess (%) | Reference |
|---|---|---|---|---|
S. marcescens MG1 | Deletion of slaC and gidA | 3R-AC | / | (8) |
K. pneumoniae | Deletion of budC and acoABCD | 3R-AC | 96 | (9) |
C. glutamicum CGR6 | Block the reduction of AC to BD, weaken the TCA cycle and insert more alsSD operon copies into the host genome | 3R-AC | 95 | (10) |
L. lactis | Deletion of ldh, ldhB, ldhX, pta, adhE, and butBA; and overexpression of the lactose-utilization pathway | 3S-AC | / | (11) |
S. marcescens | Deletion of slaC and overexpression of bdhA | (2R,3R)-2,3-BD | / | (12) |
Enterobacter cloacae sp. | Deletion of ptsG, ldh, frdA, adh and overexpression of Galp | (2R,3R)-2,3-BD | > 97.5 | (13) |
P. polymyxa ZJ-9 | Deletion of DudA | (2R,3R)-2,3-BD | 99 | (14) |
K. oxytoca ΔldhA ΔpflB | Deletion of budC and expression of bdhA from P. polymyxa | (2R,3R)-2,3-BD | 92 | (15) |
S. cerevisiae | Deletion of PDC and overexpression of MTH1, cytoILV2, ALSD and BDH | (2R,3R)-2,3-BD | 100 | (16) |
B. licheniformis ΔbudC | Deletion of budC | (2R,3R)-2,3-BD | 99.4 | (17) |
B. licheniformis Δgdh | Deletion of gdh | meso2,3-BD | 99.2% | (17) |
B. subtilis | Deletion of bdhA, acoA, pta, Idh, and overexpression of budC, alsS, alsD | meso2,3-BD | 100 | (18) |