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Table 1 Predicted massess of epidermin-like lanthipeptides produced in this study, compared to those found in ESI–MS analyses

From: Use of the mCherry fluorescent protein to optimize the expression of class I lanthipeptides in Escherichia coli

Peptide

Dehydrated

Predicted mass

Observed mass

Charge

Difference (Da)

Fig.

(m/z)

(Da)

(m/z)

(Da)

Clausin

7×

1054.937

2107.860

1056.403

2110.791

+ 2

2.931

Fig. 10A

 

6×

1063.937

2125.860

1064.922

2127.829

+ 2

1.969

 
 

5×

1072.937

2143.860

1073.920

2145.825

+ 2

1.965

 

Epidermin

5×

722.344

2164.010

722.654

2164.940

+ 3

0.940

Fig. 10B

 

4×

728.344

2182.010

729.338

2184.992

+ 3

2.982

 
  1. The charges and the mass error shown as the absolute difference in Daltons are also indicated
  2. Mass (Da) = m/z * 2 − 2 * 1.007276 when carrying 2+ charged ions or Mass (Da) = m/z * 3 − 3 * 1.007276 when carrying 3+ charges
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Microbial Cell Factories

ISSN: 1475-2859

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