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Fig. 8 | Microbial Cell Factories

Fig. 8

From: Use of the mCherry fluorescent protein to optimize the expression of class I lanthipeptides in Escherichia coli

Fig. 8

Antimicrobial activity and cleavage optimization of mCherry-PreLanthipeptides using hNisP. A mCherry-PreClausA purified from strain ClausSTldhT7 and B mCherry-PreEpiA purified from strain EpiSTldhT7 after cleavage for 20 h at 26 °C respectively. hNisP was added at concentrations 0, 1.1, 15, 120 and 300 ng/µL. After cleavage, 50 µL of mCherry fused Pre-Lanthipeptide was spotted from each cleavage reaction against L. sakei

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Microbial Cell Factories

ISSN: 1475-2859

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