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Fig. 7 | Microbial Cell Factories

Fig. 7

From: Use of the mCherry fluorescent protein to optimize the expression of class I lanthipeptides in Escherichia coli

Fig. 7

Antimicrobial activity and optimization results for mCherry-PreNisA expressed in M9 minimal media, LB, BHI, TBG (TB containing 0.5% glycerol) and TB-G (TB without 0.5% glycerol). Antimicrobial activity of IMAC-purified mCherry-PreNisA produced by cultures that were A induced (On) with 0.15 mM IPTG and 0.5% glycerol (v/v) and those that were kept B un-induced (off). Uncleaved (UC) and hNisP cleaved (C) activity results are shown for all samples tested. Antimicrobial activity was assessed using an agar well diffusion assay against L. sakei

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Microbial Cell Factories

ISSN: 1475-2859

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