Fig. 6

Fluorometric intensity of E. coli BL21 expressing mCherry-NisA in M9 minimal media, LB, BHI, TBG (TB containing 0.5% glycerol), TB-G (TB without 0.5% glycerol). A In vivo fluorometric measurements of cultures induced with 0.15 mM IPTG and 0.5% glycerol (v/v). B In vivo fluorometric measurements of un-induced cultures. Means (SEM) from three independent (n = 3) cultures are shown with standard deviations indicated by error bars. Statistical differences were determined by two-way ANOVA (P < 0.05, P < 0.01 and P < 0.001). Different single-letter combinations indicate which groups have significance at different time points and should be interpreted as (a) vs (a) = no difference; (a) vs (b) = different; (b) vs (c) = different and (c) vs (d) different