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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Use of the mCherry fluorescent protein to optimize the expression of class I lanthipeptides in Escherichia coli

Fig. 4

Antimicrobial activity of mCherry-NisA at various hNisP cleavage concentrations. Antimicrobial activity of A mCherry-NisA purified from strain NisSTldhSTldh and B mCherry-NisA purified from stain NisSTldhT7. mCherry-NisA cleavage was performed at 26 °C for (1) 4 h and (2) 20 h, respectively. Cleavage was assessed using a spot plate assay against L. sakei. hNisP was added at concentrations 0, 0.01, 1.1, 15, 120, and 300 ng/µL. Post cleavage, 50 µL of mCherry-NisA was spotted from each cleavage reaction

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Microbial Cell Factories

ISSN: 1475-2859

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