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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Use of the mCherry fluorescent protein to optimize the expression of class I lanthipeptides in Escherichia coli

Fig. 1

Overview of lanthipeptide expression strains constructed in this study. A The NisSTldhT7 mCherry-NisA heterologous expression strain was co-transformed with plasmids pRSTcnA*nB and pACYCNisCi. B Strain NisSTldhSTldh was co-transformed with pRSScnA*nB and pACYCNisCi. The Pre-ClausA and Pre-EpiA-producing strains were co-transformed with pRSTccA*cB (for clausin) or pRSTceA*cB (for epidermin) and plasmids pClausCi and pClausDi to yield strains C ClausSTldhT7 and EpiSTldhT7, respectively. Relevant promoter and gene combinations are indicated in D. Figure created in biorender (http://biorender.io)

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Microbial Cell Factories

ISSN: 1475-2859

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