Table 3 Primers used in the study. Underline indicates presence of restriction site
Name | Sequence | Purpose |
|---|---|---|
A-NcoI-F | ATACCATGGAATTATCGCTTCGGATAACTC | Creating plasmid pAD-IntE1-TEF |
A-BamHI-R | GCTGGATCCCTCATTTGAATATTTGCTACTAC | |
IntE1-1-BamHI-F | ATAGGATCCGAGGCGACACTGTTGATTGC | |
IntE1-1-NcoI-R | ATTCCATGGCGCGAAAGTCCACTAATGGC | |
IntE1-2-SphI-F | TATGCATGCGCAGCAGAGGATAGTGCTTGTG | |
IntE1-2-HindIII-R | GATAAGCTTGCGTTCAGAGGGCTTTTGGAGAC | |
hrGFP-AscI-F | TATGGCGCGCCATGGTGAGCAAGCAGATC | Creating plasmid pAD-IntE1-TEF-GFP |
hfGFP-NheI-R | GCAGCTAGCCTGCAGAATTCCTATTAC | |
Hog1-AscI-F | AATGGCGCGCCTAACATGGCGGACTTTATC | Creating plasmid pAD-Inte1-TEF-HOG1 |
Hog1-Nhe1-R | AGAGCTAGCACATTGTTTGGGTGTTTACTG | |
pp-IntE1 | GACACGATCCAGCGATAG | Testing an integration of cassettes into IntE1 region |
IntE1-2-col-R | GAGGGCTGTATGCTTGTC | |
TEF-col-R | CGCTACTGGGTCAATTTGG | |
p4928-SalI-F | ATTGTCGACCAAATACCAGCGATTAGTCAC | Creating yl-Ypd1 deletion cassette |
p4928-ApaI-R | ATAGGGCCCAGATGTGCCTCTTCGATTTC | |
t4928-NotI-F | ATAGCGGCCGCTTTGCCAAATGAACAACTTCC | |
t4928-PmeI-R | CTGGTTTAAACTCACCGTGTTAATGTTGACTC |