Table 2 Summary of investigation of activation of yl-Hog1
Transformed strains | ||||
|---|---|---|---|---|
AJD IntE1-GFP ura- | AJD IntE1-GFP hog1∆ ura- | AJD IntE1-GFP hog1∆ ypd1∆ ura- | ||
obtained strains | ||||
Plasmids used for transformation | pUC-Ura-ptC0428 (deletion of Ypd1) | AJD IntE1-GFP ypd1∆ FAILED (No deletion transformant found) | AJD IntE1-GFP hog1∆ ypd1∆ SUCCEED | - |
pAD-IntE1-TEF-HOG1 (Overexpression of Hog1) | AJE HOG1 SUCCEED | AJE hog1∆ HOG1 SUCCEED | AJE hog1∆ ypd1∆ HOG1 FAILED (No colonies after transformation) | |
pAD-IntE1-TEF-HOG1- K49R (Overexpression of Hog1-49) | AJE HOG1-49 SUCCEED | AJE hog1∆ HOG1-49 SUCCEED | AJE hog1∆ ypd1∆ HOG1-49 SUCCEED | |
pAD-IntE1-TEF-HOG1-T171A-T173A (Overexpression of Hog1-171/173) | AJE HOG1-171/173 SUCCEED | AJE hog1∆ HOG1-171/173 SUCCEED | AJE hog1∆ ypd1∆ HOG1-171/173 SUCCEED | |