Table 1 Bacteria and yeast strains used in the study
Strain | Plasmid, genotype | Source |
|---|---|---|
E. coli strains | ||
DH5α | F− endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK-mK+), λ− | [31] |
DH5α | pAD-TEF | [21] |
DH5α | pUB4-Cre1(JME547) | [32] |
DH5α | pQE80-pt-Hog1Ura | [14] |
DH5α | pUC-Ura | [33] |
DH5α | phrGFP II-C | Agilent |
DH5α | pUC57-Hog1-K49R | Genewiz |
DH5α | pUC57-Hog1-T171A-Y173A | Genewiz |
DH5α | pAD-IntE1-TEF | This study |
DH5α | pAD-IntE1-TEF-GFP | This study |
DH5α | pAD-IntE1-TEF-HOG1 | This study |
DH5α | pAD-IntE1-TEF-HOG1-K49R | This study |
DH5α | pAD-IntE1-TEF-HOG1-T171A-T173A | This study |
DH5α | pUC-Ura-p-C04928 | This study |
DH5α | pUC-Ura-pt-C04928 | This study |
Y. lipolytica strains | ||
AJD | MATA, A101: ura3-302 | [34] |
AJD | hog1∆ | [14] |
AJD | hog1∆ ura- | [14] |
AJD | IntE1-GFP | This study |
AJD | IntE1-GFP ura- | This study |
AJD | IntE1-GFP hog1∆ | This study |
AJD | IntE1-GFP hog1∆ ura- | This study |
AJE | ura+, IntE1 | This study |
AJE | HOG1 | This study |
AJE | HOG1-49 | This study |
AJE | HOG1-171/173 | This study |
AJE | hog1∆ HOG1 | This study |
AJE | hog1∆ HOG1-49 | This study |
AJE | hog1∆ HOG1-171/173 | This study |
AJD | IntE1-GFP hog1∆ ypd1∆ | This study |
AJD | IntE1-GFP hog1∆ ypd1∆ ura- | This study |
AJD | IntE1-GFP hog1∆ ypd1∆ HOG1-49 | This study |
AJD | IntE1-GFP hog1∆ ypd1∆ HOG1-171/173 | This study |