Table 1 Strains and plasmids used in this study
Strain or plasmid | Genotype or phenotype | Source or reference |
|---|---|---|
Strains | ||
E. coli S17 | RecA thi pro hsdR− M+ RP4, SmR AmpR KanR | This lab |
NX02 | Wild-type strain, CmR | This work |
NXdE | Strain NX02 (ΔssB) | This work |
NXdE I | Strain NX02 (ΔssB) harboring vector pBTxylBxylC | This work |
NXdE II | Strain NX02 (ΔssB) harboring vector pBTxylBxylC kdpgA | This work |
NXdE III | Strain NX02 (ΔssB) harboring vector pBTxylBxylCyagE | This work |
NXdE IV | Strain NX02 (ΔssB) harboring vector pBTxylBxylCyjhH | This work |
NXdE V | Strain NX02 (ΔssB) harboring vector pBTxylBxylCgarL | This work |
Plasmids | ||
pLO3 | 4937-bp suicide vector, tetR | |
pLO3ΔssB | pLO3 derivative carrying upstream and downstream fragment of ssB | This work |
pBT | A vector from pBBR1mcs-2, and the promoter has been changed from Plac to Ptac | This work |
pBT-BC | pBT derivative expressing xylBC, xylBC refers to xylB, and xylC | This work |
pBT-BCA | pBT derivative expressing xylBCA, xylBCA refers to xylB, xylC, and kdpgA | This work |