Optimization of hydrogen production in Enterobacter aerogenes by Complex I peripheral fragments destruction and maeA overexpression

Table 3 The production of metabolites and hydrogen after 20 h anaerobic fermentation in serum bottles of Ea and gene expressing strains

Strains	IAM1183	IAM1183-P	IAM1183-G	IAM1183-GP	IAM1183-EF	IAM1183-EFP	IAM1183-EFG	IAM1183-EFGP
Initial glucose (mM)	83.33	83.33	83.33	83.33	83.33	83.33	83.33	83.33
Residual sugar (mM)	38.99 ± 2.83	57.98 ± 1.05	37.65 ± 3.96	54.80 ± 0.97	33.73 ± 1.85	52.78 ± 1.32	36.53 ± 6.37	53.48 ± 2.37
Formic acid (mM)	14.01 ± 0.28	0	13.44 ± 0.27	0	15.58 ± 0.42	10.34 ± 0.27	14.9 ± 0.27	0
Lactic acid (mM)	1.02 ± 0.09	2.31 ± 0.01	3.90 ± 1.06	5.79 ± 0.10	3.26 ± 0.24	3.40 ± 0.32	2.87 ± 0.24	4.66 ± 0.01
Acetic acid (mM)	4.95 ± 0.53	1.57 ± 0.30	4.97 ± 0.09	2.62 ± 0.40	3.85 ± 0.43	4.47 ± 0.52	3.57 ± 0.92	1.53 ± 0.02
Citric acid (mM)	0.65 ± 0.10	0.57 ± 0.05	0.86 ± 0.04	0.53 ± 0.02	0.88 ± 0.05	0.15 ± 0.01	0.89 ± 0.03	0.79 ± 0.03
Acetoin (mM)	0.52 ± 0.16	0.49 ± 0.95	0.52 ± 0.16	0.72 ± 0.46	0.52 ± 0.16	0.86 ± 0.84	0.78 ± 0.10	0.59 ± 0.11
2,3-Butanediol (mM)	0	0	1.56 ± 0.31	0	1.13 ± 0.12	0	1.1 ± 0.22	0
Ethanol (mM)	2.72 ± 0.09	2.82 ± 0.05	2.66 ± 0.07	2.86 ± 0.88	2.36 ± 4.28	2.98 ± 0.92	1.58 ± 1.91	2.88 ± 0.57
H₂ yield (mol/mol glucose)	1.34 ± 0.21	0.79 ± 0.19	1.61 ± 0.03	0.94 ± 0.04	1.66 ± 0.05	1.34 ± 0.21	1.54 ± 0.04	1.41 ± 0.03

ISSN: 1475-2859