Table 2 The production of metabolites and hydrogen after 20Â h anaerobic fermentation in serum bottles of Ea and gene deficient strains
Primer for gene manipulation | Sequence (5′ to 3′) | Primer for gene qPCR verification | Sequence (5′ to 3′) |
|---|---|---|---|
NuoG-F | CCCATGCTATCAACGGGATTCAGCCGAACCTGCTTAAGACGCGCTGGTAAAATTAACCCTCACTAAAGGGCG | T7 | TAATACGACTCACTATAGG |
NuoG-R | TTGAGGATGCTTAACAGAATGTCGATGAGATCCGGTGTTAACCAACTCATTAATACGACTCACTATAGGGCTC | T7-TER | GCTAGTTATTGCTCAGCGG |
NuoEF-F | TCGTTTATCTGGGTAGTATCGATTTTGTTATGTCAGACGTG GACCGCTAAAATTAACCCTCACTAAAGGGCG | recA-f | ATTCTTTACGGCGAAGGTATCAA |
NuoEF-R | TCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATCGTAGCCATTAATACGACTCACTATAGGGCTC | recA-r | CGGGTTATCTTTCAGCCACGA |
NuoEFG-F | TCGTTTATCTGGGTAGTATCGATTTTGTTATGTCAGACGTG GACCGCTAAAATTAACCCTCACTAAAGGGCG | QmaeA-F | GGTTCTGGTAAGAGATAAAC |
NuoEFG-R | TTGAGGATGCTTAACAGAATGTCGATGAGATCCGGTGTTAACCAACTCATTAATACGACTCACTATAGGGCTC | QmaeA-R | TCTGGTAAACAATCATCTTG |
PFL-F | CAGCACTATGCTCAGAGCAAAGGTTTAACGGCTACTTTACGAGGATAAACTCAATTAACCCTCACTAAAGGGCG | Qadh-f | GAAGGCAGCCGTTGTTACCC |
PFL-R | GAGCGGTGAATCCGTAGCCCGGCTAAGCGCAGCGCCAGCCGGGGGTTTTACTAATACGACTCACTATAGGGCTC | Qadh-r | CATACGCCACAGCACTCCATTT |
G-F | GGGACTGACTTCCTGACCG | Qbddh-f | CACCGTGAACGCCTACTGCC |
G-R | CAGCCGACACGGTTAGGT | Qbddh-r | ACCAGGCTCGCCACATCGTC |
EF-F | GACTGCTATACCCGCGTGATG | Qfdh-f | CGTCCTGCAATACTTCGAGATG |
EF-R | CGTCGTCGATAGAGATAAAGG | Qfdh-r | GAACTTCAGTTTCGACAGCGATT |
EFG-F | GACTGCTATACCCGCGTGATG | Qldh-f | ACCAAAGCACGCAAGGTAATG |
EFG-R | CAGCCGACACGGTTAGGT | Qldh-r | CCGTATCGAGGTCCACAAGGA |
PFL-F | TAGGAGCTCTGAGCCGTTTATGAATCCTG | Qmdh-f | GCTGATGTGGTGCTGATTTCC |
PFL-R | TGCTCTAGATACTGCTTGGCGTCATTG | Qmdh-r | GGCTTGCGGGCAGGTTTT |
Check(cm)-F | AGTGCCAAGCTTGCATGCCT | Â | Â |
Check(cm)-R | AGTATACACTCCGCTAGCGC | Â | Â |
MaeA-F | CCGGAATTCAATGTTTCTTTTGACTACAT | Â | Â |
MaeA-R | CCCAAGCTTCTACAGCGATGAACGCTTATAGGAG | Â | Â |