Table 1 Primers were designed for recombinant plasmid construction in this study
Strain or plasmid | Genotype and relevant characteristics | Source or literature |
|---|---|---|
IAM1183 | E. aerogenes IAM1183 | This study |
IAM1183-G | ΔnuoG | This study |
IAM1183-EF | ΔnuoEF | This study |
IAM1183-EFG | ΔnuoEFG | This study |
IAM1183-P | Δpfl | This study |
IAM1183-GP | ΔnuoG/Δpfl | This study |
IAM1183-EFP | ΔnuoEF/Δpfl | This study |
IAM1183-EFGP | ΔnuoEFG/Δpfl | This study |
IAM1183/M | carrying pET-28a- maeA | This study |
IAM1183-G/M | ΔnuoG ,carrying pET-28a- maeA | This study |
IAM1183-EF/M | ΔnuoEF ,carrying pET-28a- maeA | This study |
IAM1183-EFG/M | ΔnuoEFG ,carrying pET-28a- maeA | This study |
IAM1183-P/M | Δpfl, carrying pET-28a- maeA | This study |
IAM1183-GP/M | ΔnuoG/Δpfl, carrying pET-28a-maeA | This study |
IAM1183-EFP/M | ΔnuoEF/Δpfl, carrying pET-28a- maeA | This study |
IAM1183-EFGP/M | ΔnuoEFG/Δpfl, carrying pET-28a- maeA | This study |
E. coli DH5α | F-, φ 80dlacZ ΔM15, Δ (lacZYA -argF) U169, deoR, recA1, endA1, hsdR17 (rK-, mK+), phoA, supE44, λ-, thi − 1, gyrA96, relA1 | TaRaKa |
E. coli BL21(DE3) | F − ompT hsdSB(rB − mB − ) galdcm(DE3) | TaRaKa |
pET-28a | N-His, N-Thrombin, N-T7, C-His, Km | TaRaKa |
pKD46 | Cm | This lab |
pCP20 | Cm | TaRaKa |
FRT | Km, FRT site | This study |