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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Fused expression of Sm1-Chit42 proteins for synergistic mycoparasitic response of Trichoderma afroharzianum on Botrytis cinerea

Fig. 5

Cellular localization analysis of Sm1FITC., Chit42FITC, and SCfFITC on hyphae of B. cinerea. FITC labeled protein was quantified by the Bradford method and 2.5 mΜ protein was added into the hyphae germinated from 600 µl B. cinerea mycelium pre-inoculated in a glass paper slide at 28 ℃ for 12 h. FM4-64 was used as B. cinerea cell membrane stains. The fluorescence intensity of FITC-labeled proteins and B. cinerea cell membrane were measured by ImageJ software

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