Fig. 2
Chemotropism of T. afroharzianum engineered strains. (A) Schematic representation of the plate chemotropism assay. Test compound and solvent control are applied to opposite sides of a Petri dish containing a layer of water agar with 2 × 105 /ml conidia spore of T. afroharzianum wild-type (T30), OE:Sm1, OE:Chit42, and OE:SCf at a distance of 0.5 cm from the central scoring line; (B) Directed growth of germ tubes after exposure to the indicated compounds. Letters represent conditions with significant differences according to the post hoc ANOVA Fisher’s test (p < 0.05); (C) Germination of conidia spore of T. afroharzianum over time. Germ tube emergence sites are visualized by inserted the GFP protein into T. afroharzianum wild-type (T30), OE:Sm1, OE:Chit42, and OE:SCf. DIC, differential interference contrast. Scale bar, 50 µm; (D) Mycelium chemotropism of T. afroharzianum towards cellulose (control solvent), B. cinerea cell wall, and colloidal chitin. The left filter slice is B. cinerea cell wall or colloidal chitin; The right filter slice is cellulose