Table 1 Summary of genome editing efficiency of the transformants by using the CRISPR/Cas9, CRISPR/Cas12a-RVR, and CRISPR/Cas12a-RR systems
Host straina | Target genes | Elements in co-transformation | PAM sequences | No. of analysed Transformants | Gene disruption efficiency (%) |
|---|---|---|---|---|---|
MtWT | Mtstk-12 | Cas9 + sgRNA + donor | TGG | 20 | 75 |
Cas12a-RVR + crRNA + donor | TTTC | 22 | 82 | ||
Cas12a-RR + crRNA + donor | TTCG | 22 | 77 | ||
MtWT | Mtap3m | Cas9 + sgRNA + donor | AGG | 18 | 61 |
Cas12a-RVR + crRNA + donor | TATA | 20 | 70 | ||
Cas12a-RR + crRNA + donor | TTCC | 19 | 68 | ||
MtWT | Mtdsc-1 | Cas9 + sgRNA + donor | CGG | 20 | 95 |
Cas12a-RVR + crRNA + donor | TATG | 20 | 100 | ||
Cas12a-RR + crRNA + donor | TTCA | 20 | 95 | ||
MtWT | Mtsah-2 | Cas9 + sgRNA + donor | CGG | 20 | 75 |
Cas12a-RVR + crRNA + donor | TATC | 20 | 90 | ||
Cas12a-RR + crRNA + donor | TCCC | 20 | 85 |