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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Development of the thermophilic fungus Myceliophthora thermophila into glucoamylase hyperproduction system via the metabolic engineering using improved AsCas12a variants

Fig. 5

Amylolytic phenotypes analysis of the generated hyper-secretion mutants and MtWT strain. (A) Assays of total secreted protein in culture supernatants for all M. thermophila strains after 7-days culture in 2% starch medium supplemented with 0.75% yeast extract. (B) The sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the proteins secreted by M. thermophila strains after 7 days on starch medium supplemented with 0.75% yeast extract. Extracellular proteins were collected from the culture supernatants secreted by the M. thermophila strains and proteins contained within an equivalent of 30 µL culture supernatants was resolved on a precast NuPAGE™ 4–12% gradient Bis-Tris gel, which was the commercially available NuPAGE electrophoresis system obtained from Thermo Fisher Scientific (Cat. No. NP0335BOX). Gels were stained using Coomassie blue. (C) The activities of glucoamylase and α-amylase in cultures supernatants of the M. thermophila strains after 7 days of cultivation in starch medium. Values are means ± SD (n = 3 repeats)

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Microbial Cell Factories

ISSN: 1475-2859

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