Skip to main content
Fig. 4 | Microbial Cell Factories

Fig. 4

From: Development of the thermophilic fungus Myceliophthora thermophila into glucoamylase hyperproduction system via the metabolic engineering using improved AsCas12a variants

Fig. 4

Construction of the glucoamylase hyperproducing strains via genetically engineering the gene expression, secretory pathway and the SREBP pathway by our CRISPR/Cas9-assisted marker recycling system. (A) Schematic illustration of the constructed strains MtGM7, MtGM8, MtGM10 and MtGM12 through iterative multiplex genome editing in consecutive steps in this study, including deleting four target genes Mtstk-12, Mtap3m, Mtdsc‑1 and Mtsah‑2 and overexpressing the genes Mtamy1 and Mtpga3. (B) Microscopic fluorescence imaging of conidia of the obtained MtGM12 mutant. Scale bar represents 10 μm

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us