Fig. 3

Hyper-production of amylolytic enzymes in M. thermophila due to deletion of two SREBP pathway components Mtdsc‑1 and Mtsah‑2. (A-B) Assays for protein concentration and amylase activities of culture supernatants of the ∆Mtdsc-1, ∆Mtsah-2 and MtWT strains after 4-days and 5-days shake-flask fermentation on starch. (C) SDS-PAGE analysis of secreted protein of the ∆Mtdsc-1, ∆Mtsah-2 and MtWT strains after 5-days of incubation on starch. (D) Transcript levels of the genes MtglaA and Mtamy1 in the ∆Mtdsc-1, ∆Mtsah-2 and MtWT under starch condition by RT-qPCR analysis. (E) Transcript levels of the genes MtglaA and Mtamy1 in the ∆Mtdsc-1, ∆Mtsah-2 and MtWT after 24–72 h growth on starch medium. Error bars indicate the SD from three replicates