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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Development of the thermophilic fungus Myceliophthora thermophila into glucoamylase hyperproduction system via the metabolic engineering using improved AsCas12a variants

Fig. 2

Deletion of Mtap3m increased the production of starch-degrading enzymes. (A) Assays for secreted protein concentration of the ΔMtap3m mutants and MtWT strain in 2% starch medium after 4 and 5 days starch culture. (B) SDS-PAGE analysis of secreted protein of the ΔMtap3m and MtWT strains after 5-days of incubation. (C) Glucoamylase activity assay in supernatants of 4- and 5-day starch-grown cultures of the ΔMtap3m and MtWT strains. (D) Expression levels of the genes MtglaA and Mtamy1 in the ΔMtap3m compared to MtWT under starch condition by RT-qPCR analysis. Error bars indicate the SD from three replicates

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Microbial Cell Factories

ISSN: 1475-2859

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