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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Development of the thermophilic fungus Myceliophthora thermophila into glucoamylase hyperproduction system via the metabolic engineering using improved AsCas12a variants

Fig. 1

Effects of Myceliophthora thermophila Mtstk-12 deletion on the amylolytic enzymes production. (A) Schematic view of the Mtstk-12 disruption in M. thermophila using the CRISPR-Cas9 system. (B) Extracellular protein levels in culture supernatants of the ∆Mtstk-12 mutants and wild-type MtWT strains after 4-day and 5-day incubating in starch. (C) SDS-PAGE analysis of the proteins secreted by M. thermophila strains after 5 days on starch medium. (D) Glucoamylase activity assay in the culture supernatants for the M. thermophila strains. (E) Transcript levels of the key glucoamylase gene MtglaA and α-amylase gene Mtamy1 in the ∆Mtstk-12 mutant relative to MtWT strain on starch. The data are normalized to the expression of the MtWT strain at 24 h for each tested gene, with actin gene (Mycth_2314852) expression levels used as an endogenous control in all samples. Error bars indicate the SD from three replicates

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Microbial Cell Factories

ISSN: 1475-2859

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