Fig. 6
The pentose assimilation and pentose phosphate pathways of R. toruloides with abundance changes in Pnt1 mutants. Arrow size is proportional to the log2-fold change (LFC) between OE-Pnt1 and ∆Pnt1 strains on xylose plus glycerol, except for RTO4_12976 which was undetected in ∆Pnt1. In cases where multiple proteins are assigned to a reaction, the lowest magnitude fold change is represented. *XXXX = Protein for which high throughput fitness data confirmed function in the pathway but was inconclusive as to which enzymatic reaction(s) are mediated. Metabolite abbreviations: Glu6P d-glucose-6-phosphate, 6P-GCL 6-phospho-d-glucono-1,5-lactone, 6P-GCA 6-phospho-d-gluconate, Rib5p d-ribulose 5-phosphate, aRib5p alpha-d-ribulose-5-phosphate, Fru6P d-fructose-6-phosphate, X5P xylulose-5-phosphate, Fru16P d-fructose-1-6-bisphosphate, Sed17P sedoheptulose 1,7-bisphosphate, Sed7P sedoheptulose 7-phosphate, GA3P glyceraldehyde 3-phosphate, DHAP dihydroxyacetone phosphate, Acetyl-P acetyl phosphate, Ery4P d-erythrose 4-phosphate, G3P glycerol-3-phosphate