Table 1 Effect of replacement of the promoter fused to the phaC1STQK-AB operon in pTV118NpctphaC1ps(ST/QK)AB plasmid on P(LA-co-3HB) production
Promoter | P(LA-co-3HB) quantification method | Glucose-supplemented LB medium | Xylose-supplemented LB medium | |||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Nile red staining | GC-FID | |||||||||||||||||||||||
Gene function | A430 | S.D. | Ratio | 3HB (g/L) | S.D. | Ratio | LA (g/L) | S.D. | Ratio | Total (g/L) | Ratio | LA composition (%) | S.D. | 3HB (g/L) | S.D. | Ratio | LA (g/L) | S.D. | Ratio | Total (g/L) | Ratio | LA composition (%) | S.D. | |
Original promoter | ||||||||||||||||||||||||
phaC | PHA synthase | 1.28 | 0.21 | 1.00 | 2.50 | 0.35 | 1.00 | 0.20 | 0.04 | 1.00 | 2.70 | 1.00 | 8 | 0.00 | 2.82 | 0.23 | 1.00 | 0.82 | 0.03 | 1.00 | 3.64 | 1.00 | 23 | 0.01 |
Stationary phase inducible promoter | ||||||||||||||||||||||||
dps | Stationary phase nucleoid protein | 2.99 | 0.09 | 2.33 | 7.66 | 0.36 | 3.06 | 1.12 | 0.18 | 5.62 | 8.78 | 3.25 | 14 | 0.03 | 2.55 | 0.11 | 0.90 | 0.82 | 0.11 | 0.99 | 3.37 | 0.92 | 24 | 0.02 |
sodC | Superoxide dismutase | 2.23 | 0.28 | 1.74 | 5.16 | 0.92 | 2.06 | 0.56 | 0.19 | 2.82 | 5.72 | 2.12 | 12 | 0.02 | 2.17 | 0.58 | 0.77 | 0.43 | 0.05 | 0.53 | 2.61 | 0.72 | 17 | 0.01 |
yliH | Regulator of biofilm formation | 2.05 | 0.33 | 1.60 | 5.75 | 0.49 | 2.30 | 0.75 | 0.08 | 3.77 | 6.50 | 2.41 | 13 | 0.01 | 3.34 | 0.37 | 1.19 | 2.25 | 0.28 | 2.74 | 5.59 | 1.54 | 40 | 0.02 |
gadB | Glutamate decarboxylase B | 1.91 | 0.08 | 1.49 | 5.27 | 0.03 | 2.11 | 0.35 | 0.04 | 1.74 | 5.61 | 2.08 | 8 | 0.01 | 3.37 | 0.26 | 1.19 | 1.03 | 0.03 | 1.25 | 4.39 | 1.21 | 23 | 0.02 |
treA | Trehalase | 1.89 | 0.05 | 1.48 | ||||||||||||||||||||
yceP | Regulator of biofilm formation | 1.66 | 0.11 | 1.29 | ||||||||||||||||||||
osmC | Osmotically inducible peroxiredoxin | 1.56 | 0.18 | 1.22 | ||||||||||||||||||||
osmB | Osmotically inducible lipoprotein | 1.52 | 0.13 | 1.18 | ||||||||||||||||||||
yciF | Osmotically inducible protein | 1.36 | 0.18 | 1.06 | ||||||||||||||||||||
slp | Starvation lipoprotein | 1.16 | 0.26 | 0.91 | ||||||||||||||||||||
osmY | Chaperone | 1.03 | 0.30 | 0.80 | ||||||||||||||||||||
gadA | Glutamate decarboxylase A | 1.01 | 0.27 | 0.78 | ||||||||||||||||||||
fic | Adenosine monophosphate transferase | 0.85 | 0.07 | 0.66 | ||||||||||||||||||||
yjbJ | Stress response protein | 0.67 | 0.20 | 0.52 | ||||||||||||||||||||
poxB | Pyruvate oxidase | 0.61 | 0.11 | 0.48 | ||||||||||||||||||||
yiaG | Putative transcriptional regulator | 0.51 | 0.18 | 0.39 | ||||||||||||||||||||
wrbA | quinone oxidoreductase | 0.14 | 0.05 | 0.11 | ||||||||||||||||||||
csiD | Carbon starvation inducible protein | 0.05 | 0.02 | 0.04 | ||||||||||||||||||||
Constitutive promoter | ||||||||||||||||||||||||
uxuA | d-Mannonate dehydratase | 0.83 | 0.07 | 0.65 | ||||||||||||||||||||
modA | Molybdate transporter | 0.27 | 0.17 | 0.21 | ||||||||||||||||||||
glpD | Glycerol 3-phosphate dehydrogenase | 0.20 | 0.17 | 0.15 | ||||||||||||||||||||