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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Cell-growth phase-dependent promoter replacement approach for improved poly(lactate-co-3-hydroxybutyrate) production in Escherichia coli

Fig. 6

P(LA-co-3HB) production and expression level of the phaC1STQK-AB operon by replacement of the promoter fused to the phaC1STQK-AB operon in xylose-supplemented LB medium. A The bars indicate the amount of 3HB units in the polymer (black), the amount of LA units in the polymer (white), and the LA fraction (gray). B, C The x-axis shows the expression ratio of phaC mRNA to the ribosomal RNA, rrsA, quantified by RT-qPCR after 24 h of incubation. The y-axis shows the production of P(LA-co-3HB) after 48 h of incubation (B), and the LA fraction (C). pTV indicates the conventional plasmid pTV118NpctphaC1ps(ST/QK)AB. The gene name indicates the promoter replaced with the phaC promoter

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Microbial Cell Factories

ISSN: 1475-2859

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