Table 6 Plackett Burman Design matrix and experimental results
Runs | X1 | X2 | X3 | X4 | X5 | X6 | X7 | X8 | X9 | FAME conc (%), replicates | Lipase activity (U/g), replicates | Biomass (gL−1), replicates | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 1 | 2 | 3 | 1 | 2 | 3 | ||||||||||
01–03 | − 1 | − 1 | − 1 | 1 | − 1 | − 1 | 1 | − 1 | 1 | 30.46 | 32.53 | 30.07 | 29.58 | 24.38 | 25.00 | 2.59 | 2.48 | 2.53 |
04–06 | − 1 | − 1 | 1 | − 1 | − 1 | 1 | − 1 | 1 | 1 | 36.96 | 33.78 | 32.59 | 99.79 | 80.21 | 82.71 | 19.42 | 20.05 | 20.24 |
07–09 | − 1 | − 1 | 1 | − 1 | 1 | 1 | 1 | − 1 | − 1 | 28.67 | 26.99 | 26.85 | 45.42 | 41.25 | 48.75 | 8.66 | 8.38 | 7.57 |
10–12 | − 1 | 1 | − 1 | − 1 | 1 | − 1 | 1 | 1 | 1 | 41.43 | 41.21 | 40.58 | 28.33 | 33.33 | 26.04 | 2.23 | 1.83 | 2.61 |
13–15 | − 1 | 1 | − 1 | 1 | 1 | 1 | − 1 | − 1 | − 1 | 25.94 | 25.78 | 26.19 | 17.50 | 20.83 | 15.83 | 11.09 | 8.33 | 12.68 |
16–18 | − 1 | 1 | 1 | 1 | − 1 | − 1 | − 1 | 1 | − 1 | 30.32 | 30.70 | 30.23 | 43.13 | 78.33 | 63.75 | 5.55 | 4.87 | 6.32 |
19–21 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 53.24 | 51.60 | 52.81 | 42.29 | 33.13 | 48.33 | 11.20 | 9.62 | 10.14 |
22–24 | 1 | − 1 | − 1 | − 1 | 1 | − 1 | − 1 | 1 | − 1 | 0.00 | 0.00 | 0.00 | 5.42 | 6.04 | 3.75 | 2.19 | 2.44 | 2.55 |
25–27 | 1 | − 1 | − 1 | 1 | − 1 | 1 | 1 | 1 | − 1 | 39.31 | 38.23 | 38.00 | 78.33 | 97.71 | 88.75 | 13.82 | 13.60 | 14.52 |
28–30 | 1 | − 1 | 1 | 1 | 1 | − 1 | − 1 | − 1 | 1 | 0.00 | 0.00 | 0.00 | 3.54 | 4.38 | 4.58 | 5.07 | 4.39 | 5.09 |
31–33 | 1 | 1 | − 1 | − 1 | − 1 | 1 | − 1 | − 1 | 1 | 30.50 | 30.36 | 30.11 | 37.29 | 43.54 | 44.79 | 10.28 | 10.76 | 10.66 |
34–36 | 1 | 1 | 1 | − 1 | − 1 | − 1 | 1 | − 1 | − 1 | 31.86 | 32.27 | 32.12 | 46.04 | 32.92 | 33.33 | 6.21 | 6.16 | 5.57 |
37–39 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 40.33 | 40.35 | 40.40 | 30.83 | 28.33 | 28.75 | 19.47 | 21.26 | 20.33 |