From frozen cell bank to product assay: high-throughput strain characterisation for autonomous Design-Build-Test-Learn cycles

Table 1 Batch times of cultures inoculated from cryo cultures stored at −80 \(^\circ\)C and −20 \(^\circ\)C

Batch times were investigated weekly over the course of six weeks by cultivation and subsequent spline analysis of the growth curves (section Spline analysis for batch time calculcation). For each of the storage conditions and weeks, three different cryo cultures were used, each of those for inoculation of four wells to an optical density (OD) of 0.1. This leads to a number of 12 replicates per storage condition and week. A minimum error in time of 4 min was assumed since this is the cycle time used for measurements in the BioLector^®

ISSN: 1475-2859