Fig. 7

Overview of CIP strategies. In both strategies, disinfection takes place with an ethanol/propan-1-ol mixture which is removed by liquid handling in two steps. In CIP 1, the residual liquid, which was determined to be less than 10 \(\upmu\)L, is diluted by adding and removing methanol. The residual methanol (< 10 \(\upmu\)L) is passively removed by evaporation. In CIP 2, the residual disinfectant is diluted by multiple wash steps with fresh CGXII medium