Fig. 5

CIP procedure using fresh medium to remove disinfectant. Using the CIP combined with the preculture strategy from section "Step 2: Integrated preculture strategy for high reproducibility", three consecutive cultivations were performed with C. glutamicum wild-type. In detail, 12 precultures were cultivated to a backscatter threshold of 5.5 a.u.. Each of those was then used to inoculate three main culture replicates, resulting in 36 replicates overall. These were again harvested upon a threshold of 5.5 a.u.. The shaded area indicates the period of harvesting cells, disinfecting wells and performing CIP, which took about 2 h