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Fig. 4 | Microbial Cell Factories

Fig. 4

From: From frozen cell bank to product assay: high-throughput strain characterisation for autonomous Design-Build-Test-Learn cycles

Fig. 4

CIP strategy using methanol for disinfectant removal. 12 biological replicates of C. glutamicum wild-type were cultivated in a FlowerPlate®. After a first batch phase up to a backscatter of 2 a.u., the CIP takes around 11 h. A period of 24 h is used to test for sterile conditions before wells are inoculated from fresh cryo cultures at 46 h process time

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