Fig. 2

Comparison of preculture reproducibility after inoculation from different cryo cultures. a Storage in Eppendorf Tubes^® with OD 20, glycerol content of 25% (w/v), manual dilution to OD 4 with 0.9% (w/v) NaCl solution before inoculation. b Storage in MTP with OD 4, glycerol content of 25% (w/v), directly used for inoculation. c Storage in MTP with OD 8, glycerol content of 25% (w/v), addition of pre-warmed 50% (w/v) glycerol by liquid handler before inoculation. d: Storage in MTP with OD 8, glycerol content of 25% (w/v), addition of pre-warmed 0.9% (w/v) NaCl solution by liquid handler before inoculation