Table 7 Precipitation and dialysis of α-amylase enzyme using ethanol as an organic solvent
Ethanol PPT | Total protein mg/ml | Total activity (U/ml) | Specific activity (U/mg) | Purification fold |
|---|---|---|---|---|
Crude enzyme | 14.8 | 2218 | 149.8 | 1 |
0–25 | 2 | 12 | 6 | 0.043 |
25–50 | 3.3 | 31 | 9.3 | 0.062 |
50–75 | 3.9 | 30 | 7.6 | 0.050 |
75–100 | 0.8 | 4 | 5 | 0.033 |