Table 6 Precipitation and dialysis of α-amylase enzyme using ammonium sulphate
Saturation (%) | Total protein mg/ml | Total activity (U/ml) | Specific activity (U/mg) | Purification fold |
|---|---|---|---|---|
Crude enzyme | 14.8 | 2218 | 149.8 | 1 |
0–20 | 1.15 | 17 | 14.7 | 0.098 |
20–40 | 4.9 | 147 | 30 | 0.203 |
40–60 | 5.1 | 172 | 33.7 | 0.224 |
60–80 | 0.3 | 3 | 10 | 0.066 |
80–100 | 0 | 0 | 0 | 0 |