Table 4 Test factors and levels used for optimization α-amylase by CCD model showing the most significant factors that affected the production
Central composite design matrix | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Run | Factor 1 | Factor 2 | Factor 3 | Factor 4 | Response 1 | Response 2 | ||||
A:(NH4)2SO4 g/l | B:glucose g/l | C:MgSO4 g/l | D:Peptone g/l | Predicted α-mylase activity | Calculated α-amylase activity | |||||
U/ml | U/ml | |||||||||
1 | − 2 | 7.5 | 0 | 7.5 | 0 | 0.75 | 0 | 12.5 | 160 | 155 |
2 | − 1 | 10 | − 1 | 5 | − 1 | 0.5 | 1 | 15 | 158 | 158 |
3 | 1 | 15 | − 1 | 5 | − 1 | 0.5 | 1 | 15 | 195 | 190 |
4 | 1 | 15 | 1 | 10 | − 1 | 0.5 | 1 | 15 | 187.08 | 179 |
5 | − 1 | 10 | − 1 | 5 | − 1 | 0.5 | − 1 | 10 | 159.83 | 162 |
6 | 1 | 15 | − 1 | 5 | 1 | 1 | − 1 | 10 | 181.5 | 190 |
7 | 1 | 15 | 1 | 10 | 1 | 1 | − 1 | 10 | 164.42 | 160 |
8 | 0 | 12.5 | 0 | 7.5 | 0 | 0.75 | 0 | 12.5 | 249 | 240 |
9 | 0 | 12.5 | 0 | 7.5 | 0 | 0.75 | 0 | 12.5 | 249 | 240 |
10 | − 1 | 10 | − 1 | 5 | 1 | 1 | 1 | 15 | 161 | 155 |
11 | 0 | 12.5 | 0 | 7.5 | − 2 | 0.25 | 0 | 12.5 | 161 | 157 |
12 | − 1 | 10 | 1 | 10 | − 1 | 0.5 | 1 | 15 | 159.83 | 163 |
13 | 0 | 12.5 | 0 | 7.5 | 0 | 0.75 | 0 | 12.5 | 249 | 250 |
14 | 0 | 12.5 | 0 | 7.5 | 0 | 0.75 | 0 | 12.5 | 249 | 250 |
15 | 1 | 15 | 1 | 10 | 1 | 1 | 1 | 15 | 181.5 | 180 |
16 | 1 | 15 | − 1 | 5 | 1 | 1 | 1 | 15 | 196 | 196 |
17 | − 1 | 10 | 1 | 10 | − 1 | 0.5 | − 1 | 10 | 166.25 | 165 |
18 | 1 | 15 | 1 | 10 | − 1 | 0.5 | − 1 | 10 | 135 | 135 |
19 | 0 | 12.5 | 0 | 7.5 | 0 | 0.75 | 0 | 12.5 | 249 | 238 |
20 | − 1 | 10 | 1 | 10 | 1 | 1 | − 1 | 10 | 195 | 195 |
21 | − 1 | 10 | − 1 | 5 | 1 | 1 | − 1 | 10 | 197.75 | 202 |
22 | − 1 | 10 | 1 | 10 | 1 | 1 | 1 | 15 | 135 | 140 |
23 | 0 | 12.5 | 0 | 7.5 | 0 | 0.75 | 2 | 17.5 | 146 | 146 |
24 | 0 | 12.5 | 2 | 12.5 | 0 | 0.75 | 0 | 12.5 | 184 | 188 |
25 | 0 | 12.5 | − 2 | 2.5 | 0 | 0.75 | 0 | 12.5 | 197 | 197 |
26 | 0 | 12.5 | 0 | 7.5 | 0 | 0.75 | − 2 | 7.5 | 156.08 | 162 |
27 | 0 | 12.5 | 0 | 7.5 | 0 | 0.75 | 0 | 12.5 | 249 | 235 |
28 | 1 | 15 | − 1 | 5 | − 1 | 0.5 | − 1 | 10 | 146 | 146 |
29 | 0 | 12.5 | 0 | 7.5 | 2 | 1.25 | 0 | 12.5 | 189.08 | 200 |
30 | 2 | 17.5 | 0 | 7.5 | 0 | 0.75 | 0 | 12.5 | 199 | 199 |