Table 2 Test factors and levels used in optimization of α-amylase production by PBD model
Plackett–Burman design matrix | ||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Run | Factor 1 | Factor 2 | Factor 3 | Factor 4 | Factor 5 | Factor 6 | Factor 7 | Factor 8 | Factor 9 | Factor 10 | Factor 11 | Response 1 | Response 2 | |||||||||||
A:Starch g/l | B:Glucose g/l | C:Lactose g/l | D:yeast extract g/l | E:peptone g/l | F:(NH4)2 SO4 g/l | G:CaCl2 g/l | H:KCl g/l | J:MgSO4 g/l | K:CuSO4 g/l | L:Tween 80 g/l | predicted α-amylase activity | calculated α-amylase activity | ||||||||||||
U/ml | U/ml | |||||||||||||||||||||||
1 | − 1 | 5 | 1 | 5 | 1 | 5 | − 1 | 5 | 1 | 10 | 1 | 10 | 1 | 0.05 | − 1 | 0 | − 1 | 0 | − 1 | 0 | 1 | 1 | 195 | 195 |
2 | 1 | 15 | 1 | 5 | 1 | 5 | − 1 | 5 | − 1 | 5 | − 1 | 5 | 1 | 0.05 | − 1 | 0 | 1 | 0.25 | 1 | 0.05 | − 1 | 0 | 137 | 137 |
3 | − 1 | 5 | − 1 | 0 | − 1 | 0 | 1 | 10 | − 1 | 5 | 1 | 10 | 1 | 0.05 | − 1 | 0 | 1 | 0.25 | 1 | 0.05 | 1 | 1 | 152 | 152 |
4 | 1 | 15 | 1 | 5 | − 1 | 0 | 1 | 10 | 1 | 10 | 1 | 10 | − 1 | 0 | − 1 | 0 | − 1 | 0 | 1 | 0.05 | − 1 | 0 | 150 | 142 |
5 | 1 | 15 | − 1 | 0 | 1 | 5 | 1 | 10 | − 1 | 5 | 1 | 10 | 1 | 0.05 | 1 | 0.05 | − 1 | 0 | − 1 | 0 | − 1 | 0 | 88 | 88 |
6 | − 1 | 5 | 1 | 5 | − 1 | 0 | 1 | 10 | 1 | 10 | − 1 | 5 | 1 | 0.05 | 1 | 0.05 | 1 | 0.25 | − 1 | 0 | − 1 | 0 | 134 | 134 |
7 | − 1 | 5 | 1 | 5 | 1 | 5 | 1 | 10 | − 1 | 5 | − 1 | 5 | − 1 | 0 | 1 | 0.05 | − 1 | 0 | 1 | 0.05 | 1 | 1 | 90 | 90 |
8 | 1 | 15 | 1 | 5 | − 1 | 0 | − 1 | 5 | − 1 | 5 | 1 | 10 | − 1 | 0 | 1 | 0.05 | 1 | 0.25 | − 1 | 0 | 1 | 1 | 142 | 137 |
9 | − 1 | 5 | − 1 | 0 | 1 | 5 | − 1 | 5 | 1 | 10 | 1 | 10 | − 1 | 0 | 1 | 0.05 | 1 | 0.25 | 1 | 0.05 | − 1 | 0 | 130 | 130 |
10 | 1 | 15 | − 1 | 0 | − 1 | 0 | − 1 | 5 | 1 | 10 | − 1 | 5 | 1 | 0.05 | 1 | 0.05 | − 1 | 0 | 1 | 0.05 | 1 | 1 | 85 | 76 |
11 | − 1 | 5 | − 1 | 0 | − 1 | 0 | − 1 | 5 | − 1 | 5 | − 1 | 5 | − 1 | 0 | − 1 | 0 | − 1 | 0 | − 1 | 0 | − 1 | 0 | 87 | 87 |
12 | 1 | 15 | − 1 | 0 | 1 | 5 | 1 | 10 | 1 | 10 | − 1 | 5 | − 1 | 0 | − 1 | 0 | 1 | 0.25 | − 1 | 0 | 1 | 1 | 149 | 149 |