Fig. 22

P. aeruginosa biofilm stained with acridine orange/propidium iodide florescent dyes before and after treatment with either commercial or purified α-amylase enzyme. (A) CLSM images of a untreated control biofilm showing that 98% of the biofilm was live appeared as green due to acridine orange dye that stain viable bacterial cells, b purified amylase-treated biofilm presenting 83% of the biofilm was red due to dead cells, and (c) following treatment with commercial α-amylase enzyme where 80% of the biofilm was red. (B) 3D images of control and treated biofilm showing a biofilm thickness of the test strain measuring 250 um while dramatically reduced biofilms were those treated with b purified α-amylase and (c) commercial α-amylase. The 3D images were analyzed by Image j software