Skip to main content
Fig. 5 | Microbial Cell Factories

Fig. 5

From: Identification and characterization of a strong constitutive promoter stnYp for activating biosynthetic genes and producing natural products in streptomyces

Fig. 5

Applications of promoter stnYp for aureonuclemycin and YM-216391 overproduction in heterologous hosts. (A) Schematic for constructing recombinant strains in which different constitutive promoters (ermEp*, kasOp*, SP44 and stnYp) were inserted upstream of the anmB gene. (B) Schematic for constructing recombinant strains in which different constitutive promoters (ermEp*, kasOp*, SP44 and stnYp) were inserted upstream of the ymI gene. (C) Comparison of aureonuclemycin yield in recombinant strains with anmBCDE cassette driven by different promoters (ermEp*, kasOp*, SP44 and stnYp) integrated into the genome of S. albus J1074 and S. lividans TK24. (D) Comparison of YM-216391 yield in recombinant strains with ymI-BC cassette driven by different promoters (ermEp*, kasOp*, SP44 and stnYp) integrated into the genome of S. albus J1074 and S. lividans TK24. Statistical analyses were performed and marked (**, p ≤ 0.05; ***, p ≤ 0.01)

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us