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Table 2 Titer of Nanobody molecules constructed with or without VHH1 building blocks in their format

From: Optimizing expression of Nanobody® molecules in Pichia pastoris through co-expression of auxiliary proteins under methanol and methanol-free conditions

Strain

Nanobody Formats

Average titer (g l-1)

NRRL Y-11430 + RPP0 + KAR2 + PDI + HAC1

With VHH1 building blocks

2.7 (N = 150)

Without VHH1 building blocks

3.1 (N = 699)

X-33

With VHH1 building blocks

1.1 (N = 40)

Without VHH1 building blocks

1.7 (N = 199)

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Microbial Cell Factories

ISSN: 1475-2859

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