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Table 1 Titer of different Nanobody molecules according to their format and expressed in different strains

From: Optimizing expression of Nanobody® molecules in Pichia pastoris through co-expression of auxiliary proteins under methanol and methanol-free conditions

   

Average titer (g l− 1)

   

Nanobody valency

System

Strain

Average titer (g l− 1)

mono

bi

tri

tetra

penta

hexa

Methanol-based

NRRL Y-11430

1.4

(N = 54)

2.7 (N = 13)

1.8 (N = 4)

0.8 (N = 18)

1.1 (N = 17)

0.8 (N = 2)

-a

NRRL Y-11430 + RPP0 + KAR2 + PDI + HAC1

3.1 (N = 849)

2.7 (N = 314)

3.2 (N = 82)

3.2 (N = 148)

3.4 (N = 172)

3 (N = 102)

4.1 (N = 31)

Methanol-free

NRRL Y-11430

0.6

(N = 16)

-

0.4 (N = 2)

-

0.5 (N = 11)

1.4 (N = 3)

-

NRRL Y-11430 + HAC1

3.7 (N = 156)

3.4 (N = 93)

4.3 (N = 12)

4.5 (N = 12)

3.7 (N = 30)

4.6 (N = 8)

3.6 (N = 1)

  1. a - = no data available
  2. Productions were performed under generic fed-batch fermentations as described in the material and methods. Indicated titer are measured in clarified cell broth. Nanobody valency reflects the number of genetically fused Nanobody building blocks in the construct
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Microbial Cell Factories

ISSN: 1475-2859

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