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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Optimizing expression of Nanobody® molecules in Pichia pastoris through co-expression of auxiliary proteins under methanol and methanol-free conditions

Fig. 1

Evaluation of HAC1, PDI1, RPP0 and KAR2 for titer improvement of Nanobody molecule A. Productions were performed at 2 L scale, pH 6, 30 °C in complex medium with a methanol feed rate of 4 ml l− 1 h− 1. Indicated titers are measured in clarified cell broth via SDS-PAGE/Coomassie staining and quantification of bandvolume measured using ImageQuant software (Cytiva). M = Molecular weight standard

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Microbial Cell Factories

ISSN: 1475-2859

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