Table 1 Biomass (CDW), riboflavin, FMN, aminoriboflavin, roseoflavin production and dimethyltransferase specific activity of C. famata and K. phaffii transformants and control strains represented on YNB medium on 72 h of cultivation
Strain | CDW (g L-1) | Riboflavin (mg L-1) | FMN (mg L-1) | AF (mg L-1) | RoF (mg L-1) | RosA specific activity (U mg-1) |
|---|---|---|---|---|---|---|
FP | 2.8 ± 0.1 | 301.1 ± 15.2 | 5.1 ± 0.2 | ND | ND | NA |
FP/rosB | 2.7 ± 0.1 | 328.4 ± 15.4 | 3.2 ± 0.1 | 1.5 ± 0.1 | ND | NA |
BRP | 2.7 ± 0.1 | 789.3 ± 38.2 | ND | ND | ND | ND |
BRP/FMN1-rosB-rosA-rosC | 2.6 ± 0.1 | 841.3 ± 40.4 | 1.1 ± 0.05 | 1.5 ± 0.1 | ND | ND |
Y-33 | 2.1 ± 0.1 | 2.3 ± 0.1 | ND | ND | ND | ND |
Y-33/FMN1-rosB | 2.2 ± 0.1 | 4.1 ± 0.2 | 0.1 | 1.9 ± 0.1 | ND | NA |
Y-33/FMN1-rosB-rosA-rosC | 2.1 ± 0.1 | 7.8 ± 0.4 | 0.1 | 2.0 ± 0.1 | 5.0 ± 0.2 | 24.1 ± 1.2 |
Y-33 (bioreactor 165 h)* | 142.9 ± 7.0 | 388.0 ± 8.7 | ND | ND | ND | NA |
Y-33/FMN1-rosB (bioreactor 96 h)* | 130.0 ± 6.4 | 564.0 ± 27.9 | 0.9 ± 0.04 | 17.0 ± 0.8 | ND | NA |
Y-33/FMN1-rosB-rosA-rosC (bioreactor 312 h)* | 133.0 ± 6.6 | 1625.4 ± 82.1 | 1.0 ± 0.05 | 21.8 ± 1.0 | 129.6 ± 6.3 | NA |