Fig. 3
Photocatalytic hydrogen evolution assay. The deaerated mixture containing methyl viologen, L-cysteine and SH_LH U493C_pMCSG53 hydrogenase variant was irradiated using a 1000 W Xenon lamp at 10oC. After 8 h of irradiation, 200 ml of gas samples were collected from the headspace of the photoreactor and injected to the gas chromatograph, coupled with thermal conductivity detector (TCD). The amount of hydrogen was calculated at µl per litre of the reaction mixture