Table 1 Summary of data supporting pentose gene-protein-reaction rules reflected in Fig. 14

N/A

DA2DH

Figure 11A, B (***)

Fig. 12B (*)

Fig. 13 (***)

N/A

Yes

Evidence of latent DA2DH activity; evolved strain produces d-arabitol

N/A

LA2DH

Figure 7 (***)

N/A

Yes

Gene(s) encoding this function have yet to be identified

9837

DA4DH

N/A

[13]

No

Single KO shows no growth defect

8905

DA4DH

N/A

[13]

No

Single KO shows no growth defect

14368

RK

Figures 7,8 (***)

[13]

Yes

Data suggests RTO4_14368 is the only RK-encoding gene

16850

XK

N/A

[13, 15]

Yes

Inactive under tested conditions in this study,but transcribed in presence of acetate [15]

13562

XR

N/A

[13]

Yes

Function unconfirmed in this study

11882

XR

N/A

[13, 40]

Yes

In [40], 11,882 has one-tenth the activity of 9774 on d-xylose

9990

DA2DH

XDH

Figures 6F, 7, 9C, 10 (***)

Fig. 9D (*)

[13]

Yes

Cannot rule out XDH function

8988

LXR

XDH

DA4DH

Figure 3D, E (***)

Fig. 3B, 9D (*)

Fig. 3C, 9E (***)

[13]

Yes

KO metabolite excretion patterns consistent with XDH activity

9774

XR

Figure 2 (*)

[13, 40]

Yes

Reduces l-arabinose, d-xylose, glyceraldehyde

16452

LA4DH

XDH

Figure 5D (***)

Fig. 9C, D (**)

[13]

Yes

KO metabolite excretion patterns consistent with XDH activity

12974

LA4DH

XDH

N/A

[13]

No

Pseudogene; no growth defects with KO

12977

LA4DH

XDH

Figure 5D (***)

N/A

[13]

Yes

KO has slight growth defect on l-arabitol;cannot rule out XDH activity without metabolite profiling